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Assessment of ADAMTS13 activity {#Sec13} For the assay of ADAMTS13 activity, a commercial kit (MyBioSource) was used according to the manufacturer's instructions. Briefly, 50 μL of commercially available recombinant ADAMTS13 (1.5 μg/mL) was incubated with 50 μL of diluted patient serum and substrate at 37 °C for 10 min. Subsequently, the reaction was stopped by adding 100 μL of dilution buffer, and the absorbance at 405 nm was measured. The enzymatic activity of ADAMTS13 was calculated as the percentage of inhibition of substrate conversion of ADAMTS13 relative to the negative control. Statistical analysis {#Sec14} -------------------- The statistical analyses were performed using GraphPad Prism 6.0 (GraphPad Software Inc., San Diego, CA, USA). A receiver operating characteristic (ROC) curve was used to estimate the diagnostic value

 

 

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